Efficacy evaluation of the S-adenosylhomocysteine hydrolase inhibitor MSD-914 in rhesus macaques (Macaca Mulatta) challenged with Ebola virus by the intramuscular route

S Sara C. Johnston A Anthony T. Ginnetti N Nancy A. Twenhafel S Sarah L. W. Norris S Shiying Chen J Josh L. Moore C Christopher W. Boyce O Ondraya M. Frick D Dave N. Dyer D Donald J. Marsh S Stephen C. Stevens W Walter F. Knapp K Kerry L. Berrier H Hui Wan G Gregory C. Adam T Timothy J. Hartingh H Heather L. Esham J Jimmy O. Fiallos E Eugene L. Blue W Willie B. Sifford J Jonathan D. Latty H Harold L. Mills N Nazira A. Alli A Ashley E. Piper A Aimee I. Goodson J J. Matthew Meinig D David B. Olsen L Linda A. Lieberman R Rekha G. Panchal

Abstract

Ebola virus (EBOV) causes a severe and often fatal hemorrhagic fever in humans for which effective postexposure countermeasures are lacking. Herein, we describe the evaluation of an S-adenosylhomocysteine hydrolase inhibitor, MSD-914, using mouse and nonhuman primate (NHP) models of lethal EBOV. Mice were completely protected from severe disease and death at doses as low as 0.31 mg/kg/day administered orally. From the pharmacological data and a toxicokinetic study, a predicted protective dose was selected for rhesus macaques (RMs). Surprisingly, orally administered MSD-914 was unable to protect RMs at doses as high as 0.8 mg/kg/day despite providing similar exposure of the drug to the efficacious dose observed in the mouse model.

Article Details

Journal PLoS ONE
Volume / Issue Vol. 21, Issue 2
Published February 06, 2026
Pages e0340118
ISSN 1932-6203
Publisher Public Library of Science

Journal Info

PLoS ONE

Public Library of Science

ISSN: 1932-6203 Open Access Health Sciences

Authors (29)

S

Sara C. Johnston

A

Anthony T. Ginnetti

N

Nancy A. Twenhafel

S

Sarah L. W. Norris

S

Shiying Chen

J

Josh L. Moore

C

Christopher W. Boyce

O

Ondraya M. Frick

D

Dave N. Dyer

D

Donald J. Marsh

S

Stephen C. Stevens

W

Walter F. Knapp

K

Kerry L. Berrier

H

Hui Wan

G

Gregory C. Adam

T

Timothy J. Hartingh

H

Heather L. Esham

J

Jimmy O. Fiallos

E

Eugene L. Blue

W

Willie B. Sifford

J

Jonathan D. Latty

H

Harold L. Mills

N

Nazira A. Alli

A

Ashley E. Piper

A

Aimee I. Goodson

J

J. Matthew Meinig

D

David B. Olsen

L

Linda A. Lieberman

R

Rekha G. Panchal