Effects of low-temperature stress at different growth stages on rice physiology, pollen viability and yield in China’s cold region

Abstract

Low-temperature stress (LTS) is a major limiting factor for rice production in high-latitude regions. Many studies have reported the impacts of LTS on leaf photosynthesis and yield, but few of them explored the response of photosynthesis, chloroplast ultrastructure, pollen fertility, cold stress adaptation to LTS at different growth stages of rice. In this study, we conducted a two-year temperature-controlled field experiment (in 2023 and 2024) to investigate the effects of LTS at the tillering, booting, and heading stages on physiological and biochemical characteristics, plant growth, pollen fertility, and grain yield for a japonica rice cultivar (Longgeng31). The results showed that rice photosynthesis gradually decreased as the LTS temperature was decreasing and the LTS duration was increasing. The net photosynthetic rate (Pn) decreased the most at the booting stage, followed by the tillering, and the heading stages. Compared with controlled group (CK), the LTS treatment at 11.5°C for 3–10 days significantly reduced Pn by 52.2% ~ 62.7%, 85.3% ~ 93.9% and 39.3% ~ 44.9%, at the tillering stage, booting and heading stages respectively. Increasing LTS intensity and duration caused distorted chloroplast morphology and reduced plant height. The concentrations of the antioxidant and osmotic regulation systems in rice peaked after 7 days of LTS treatment, indicating that the stress response to LTS showed a trend of initially increasing and subsequently decreasing. The grain yield decreased the most under LTS at the booting stage by 59.30%−88.76% on D10, followed by the heading and tillering stages. After 10 days of exposure to LTS, the pollen viability decreased most significantly at the heading stage by 44.67%, followed by the booting and the tillering stages. These findings could provide a theoretical basis for identifying and evaluating LTS in rice under field conditions, and provide a methodological reference for the identification and monitoring of LTS in other crops, thereby holding significant practical implications.