Disease-specific B cell clones are shared between patients with Crohn’s disease
Abstract
Abstract B cells have important functions in gut homeostasis, and dysregulated B cell populations are frequently observed in patients with inflammatory bowel diseases, including both ulcerative colitis (UC) and Crohn’s disease (CD). How these B cell perturbations contribute to disease remains largely unknown. Here, we perform deep sequencing of the B cell receptor (BCR) repertoire in four cohorts of patients with CD, together with healthy controls and patients with UC. We identify BCR clones that are shared between patients with CD but not found in healthy individuals nor in patients with UC, indicating CD-associated B cell immune responses. Shared clones are present in the inflamed gut mucosa, draining intestinal lymph nodes and blood, suggesting the presence of common CD-associated antigens that drive B cell responses in CD patients.
Article Details
Authors (21)
Prasanti Kotagiri
William M. Rae
Laura Bergamaschi
Diana Pombal
Ji-Yeun Lee
Nurulamin M. Noor
Raoul S. Sojwal
Samuel J. S. Rubin
Lukas W. Unger
Sofie H. Tolmeijer
Giulia Manferrari
Rachael J. M. Bashford-Rogers
David B. Bingham
Anton Stift
Stephan Rogalla
John Gubatan
James C. Lee
Kenneth G. C. Smith
Eoin F. McKinney
Scott D. Boyd
Paul A. Lyons