Discovering Targetable Conformation of RhoA Mutant by Integrating Native Mass Spectrometry, Ultraviolet Photodissociation, and X-ray Diffraction

Haozhe Wu; Zheyi Liu; Hao Jiang; Heng Zhao; Chen Dong; Yu Lu; Shijia Zu; Yongjie Guo; Can Lai; Pan Luo; Ke Xu; Yuxin Yang; Yafang Yang; Zhongya Sun; Qingcheng Huang; Huan Xiong; Lanxin Zhou; Yipan Luo; Yuhan Zeng; Daohai Du; Zhongjie Liang; Weilie Xiao; Shan Zhao; Weiqing Zhang; Yong Tang; Chunlei Xiao; Kaixian Chen; Xueming Yang; Fangjun Wang; Cheng Luo

doi:10.1021/jacs.5c20067

Journal of the American Chemical Society

Discovering Targetable Conformation of RhoA Mutant by Integrating Native Mass Spectrometry, Ultraviolet Photodissociation, and X-ray Diffraction

H Haozhe Wu (State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine) Z Zheyi Liu (State Key Laboratory of Chemical Reaction Dynamics) H Hao Jiang H Heng Zhao (State Key Laboratory of Chemical Reaction Dynamics) C Chen Dong (State Key Laboratory of Drug Research) Y Yu Lu (School of Life Science and Technology) S Shijia Zu (State Key Laboratory of Drug Research) Y Yongjie Guo (State Key Laboratory of Chemical Reaction Dynamics) C Can Lai (State Key Laboratory of Chemical Reaction Dynamics) P Pan Luo (Beijing Frontier Research Center on Clean Energy) K Ke Xu Y Yuxin Yang (State Key Laboratory of Drug Research) Y Yafang Yang (State Key Laboratory of Drug Research) Z Zhongya Sun (State Key Laboratory of Drug Research) Q Qingcheng Huang (State Key Laboratory of Drug Research) H Huan Xiong (State Key Laboratory of Drug Research) L Lanxin Zhou (State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine) Y Yipan Luo (Zhongshan Institute for Drug Discovery) Y Yuhan Zeng (Zhongshan Institute for Drug Discovery) D Daohai Du (State Key Laboratory of Drug Research) Z Zhongjie Liang (Center for Systems Biology, Department of Bioinformatics, School of Biology and Basic Medical Sciences) W Weilie Xiao (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Pharmacy and School of Chemical Science and Technology) S Shan Zhao W Weiqing Zhang Y Yong Tang (Ensem Therapeutics, Inc., 200 Boston Ave., Medford, Massachusetts 02155, United States) C Chunlei Xiao (State Key Laboratory of Chemical Reaction Dynamics) K Kaixian Chen (State Key Laboratory of Drug Research) X Xueming Yang (State Key Laboratory of Chemical Reaction Dynamics and Dalian Coherent Light Source, Dalian Institute of Chemical Physics) F Fangjun Wang (CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics) C Cheng Luo (State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine)

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Article Details

Journal Journal of the American Chemical Society

Volume / Issue Vol. 148, Issue 11

Published March 25, 2026

Pages 11709-11718

DOI 10.1021/jacs.5c20067

ISSN 0002-7863

Publisher American Chemical Society

Journal Info

Journal of the American Chemical Society

American Chemical Society

ISSN: 0002-7863 Physical Sciences

Authors (30)

H

Haozhe Wu

State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine

Z

Zheyi Liu

State Key Laboratory of Chemical Reaction Dynamics

H

Hao Jiang

H

Heng Zhao

State Key Laboratory of Chemical Reaction Dynamics

C

Chen Dong

State Key Laboratory of Drug Research

Y

Yu Lu

School of Life Science and Technology

S

Shijia Zu

State Key Laboratory of Drug Research

Y

Yongjie Guo

State Key Laboratory of Chemical Reaction Dynamics

C

Can Lai

State Key Laboratory of Chemical Reaction Dynamics

P

Pan Luo

Beijing Frontier Research Center on Clean Energy

K

Ke Xu

Y

Yuxin Yang

State Key Laboratory of Drug Research

Y

Yafang Yang

State Key Laboratory of Drug Research

Z

Zhongya Sun

State Key Laboratory of Drug Research

Q

Qingcheng Huang

State Key Laboratory of Drug Research

H

Huan Xiong

State Key Laboratory of Drug Research

L

Lanxin Zhou

State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine

Y

Yipan Luo

Zhongshan Institute for Drug Discovery

Y

Yuhan Zeng

Zhongshan Institute for Drug Discovery

D

Daohai Du

State Key Laboratory of Drug Research

Z

Zhongjie Liang

Center for Systems Biology, Department of Bioinformatics, School of Biology and Basic Medical Sciences

W

Weilie Xiao

State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Pharmacy and School of Chemical Science and Technology

S

Shan Zhao

W

Weiqing Zhang

Y

Yong Tang

Ensem Therapeutics, Inc., 200 Boston Ave., Medford, Massachusetts 02155, United States

C

Chunlei Xiao

State Key Laboratory of Chemical Reaction Dynamics

K

Kaixian Chen

State Key Laboratory of Drug Research

X

Xueming Yang

State Key Laboratory of Chemical Reaction Dynamics and Dalian Coherent Light Source, Dalian Institute of Chemical Physics

F

Fangjun Wang

CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics

C

Cheng Luo

State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine

Discovering Targetable Conformation of RhoA Mutant by Integrating Native Mass Spectrometry, Ultraviolet Photodissociation, and X-ray Diffraction

Article Details

Journal Info

Authors (30)

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