Directional Freeze‐Casting Cryogel Loaded with Quaternized Chitosan Modified Gallium Metal–Organic Frameworks to Capture and Eradicate the Resistant Bacteria for Guided Regeneration in Infected Bone Defects
Abstract
Abstract Antimicrobial resistance and impaired bone regeneration are the great challenges in treating infected bone defects. Its recurrent and resistant nature, high incidence rate, long‐term hospitalization, and high medical costs have driven the efforts of the scientific community to develop new therapies to improve the situation. Considering the complex microenvironment and persistent mechanisms mediated by resistant bacteria, it is crucial to develop an implant with enhanced osseointegration and sustained and effective infection clearance effects. Here, a positively charged quaternized chitosan (QCS) coated gallium‐based metal–organic framework (GaMOF) is designed, to capture the antibiotic‐resistant bacteria (Methicillin‐resistant Staphylococcus aureus , MRSA) as a “captor”, and rejuvenate Methicillin (Me) via disturbing the tricarboxylic acid (TCA) cycle. Then, a radially oriented porous cryogel loaded with the Me and QCSGaMOF is fabricated by the directional freeze‐casting method. The oriented porous structure has an enhanced osseointegration effect by guiding the ingrowth of osteogenic cells. In vitro and in vivo experiments prove the advantages of as‐prepared Me/QCSGa‐MOF@Cryogel in combating resistant bacteria and guiding bone regeneration in infected bone defects.
Article Details
Authors (20)
Yihao Liu
Kai Yuan
Yixuan Lin
Fujian Provincial Key Laboratory of Polymer Materials College of Chemistry and Materials Science Fujian Normal University Fuzhou China
Yiqi Yang
Cancer Centre, Institute of Translational Medicine, Faculty of Health Sciences
Weiqing Kong
Department of Orthopedic Surgery Xuzhou Central Hospital Xuzhou Clinical School of Xuzhou Medical University 199Jiefang South Road Xuzhou 221009 P. R. China
Jing Shan
Haoyi Niu
Shanghai Key Laboratory of Orthopedic Implants Department of Orthopedic Surgery Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200011 P. R. China
Weize Kong
Shanghai Key Laboratory of Orthopedic Implants Department of Orthopedic Surgery Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200011 P. R. China
Fupeng Li
School of Biological Sciences
Xiaokun Yue
Shanghai Key Laboratory of Orthopedic Implants Department of Orthopedic Surgery Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200011 P. R. China
Yun Du
Department of Cardiology, The Fourth Affiliated Hospital of Soochow University, Cyrus Tang Medical Institute, Medical College of Soochow University
Yakun Liang
Shanghai Institute of Precision Medicine Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200125 P. R. China
Haishuang Chang
Shanghai Institute of Precision Medicine Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200125 P. R. China
Zhifeng Yu
Jinwu Wang
Guangzheng Yang
Department of Prosthodontics Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200011 P. R. China
Lingyan Cao
Joint International Research Laboratory of Metabolic and Developmental Sciences, State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University
Kai Huang
Shengbing Yang
Shanghai Key Laboratory of Orthopedic Implants Department of Orthopedic Surgery Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200011 P. R. China
Tingting Tang