Differential effects of hemodialysis modalities on circulating neutrophil extracellular traps in children on maintenance hemodialysis: a cohort study

Abstract

Abstract Flow of blood through the extracorporeal circuit during conventional hemodialysis (HD) can induce neutrophil activation, leading to inflammatory and cytokine responses. So, we evaluated the effect of modality and membrane performance of dialysis session in children on maintenance HD on neutrophil activation by measuring the level of circulating neutrophil extracellular traps (NETs). Twenty-six incident children stable on LFHD for at least 3 months were recruited along with 26 age- and sex-matched healthy controls. Pre-and post-session blood samples for complete blood count (CBC), C - reactive protein (CRP), and NETs were obtained on a mid-week session. Patients were shifted to HFHD for 3 months, followed by post-dilutional OL-HDF for additional 3 months. Blood tests were repeated at the end of each interval. Post-LFHD NETs levels were significantly higher than pre-LFHD (p = < 0.0001) and control levels (p < 0.0001). Both HFHD and OL-HDF resulted in significant reduction in the post-session NETs levels in comparison to their pre-session levels (p < 0.0001, p < 0.0001 respectively). However, OL-HDF resulted in a significant reduction of post- session NETs levels to a degree that they became comparable to control levels (p = 0.928). We conclude that children on HD have high serum levels of NETs that were significantly increased by using LFHD membranes. HFHD and OL-HDF sessions led to significant reduction in NETs reflecting their possible role in reducing the dialysis related production of NETs, however OL-HDF was superior to HFHD, as it lowered post-session NETs to levels that were comparable to that of controls.