Covalent Organic Framework Nanosheets for the Assembly of Efficient Membrane Bioreactors
Abstract
Abstract Immobilizing fragile enzymes in porous materials holds significant potential for biocatalysis but encounters challenges such as mismatched enzyme size and pore structure of host materials, along with harsh assembly conditions that can denature enzymes. Herein, we present a versatile strategy for constructing membrane bioreactors through water‐mediated, vacuum‐assisted layer‐by‐layer assembly of covalent organic framework (COF) nanosheets with enzymes. This method effectively addresses pore size limitations, preserves enzyme activity, and promotes convective transport of reactants to active sites, while shielding enzymes from harmful by‐products through rapid transport in continuous membrane catalysis. The optimized bioreactor achieves a 1018‐fold increase in relative activity compared to free enzymes in batch reactions, completing substrate conversion in just 7.95 s, and demonstrating enhanced stability.
Article Details
Authors (12)
Jingxu Han
Key Laboratory of Surface & Interface Science of Polymer Materials of Zhejiang Province School of Chemistry and Chemical Engineering Zhejiang Sci‐Tech University Hangzhou 310018 China
Zhiwei Xing
Qing Guo
School of Materials Science and Engineering, Henan Institute of Advanced Technology
Di Wu
Zhuozhi Lai
Xiaoxiao Cheng
State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials School of Chemistry Chemical Engineering and Materials Science Soochow University Suzhou P. R. China
Zhifeng Dai
Yubing Xiong
State Key Laboratory of Bio‐based Fiber Materials School of Chemistry & Chemical Engineering Zhejiang Sci‐Tech University Hangzhou 310018 P. R. China
Xiangju Meng
Department of Chemistry Zhejiang University Hangzhou 310027 China
Shengqian Ma
Department of Chemistry
Feng‐Shou Xiao
Key Lab of Biomass Chemical Engineering of Ministry of Education College of Chemical and Biological Engineering Zhejiang University Hangzhou 310027 China
Qi Sun