Comprehensive mutational profiling and clinical outcome of adults AML with NUP98 rearrangement

D Ducourneau Benoit (3CH VALENCIENNES, VALENCIENNES, France) A Arnaud Pages (4Gustave Roussy Cancer Center, Department of Biostatistics and Epidemiology, villejuif, France) S Stéphanie Struski (4Department of Hematology, University Hospital of Toulouse, Toulouse, France) M Matthieu Decamp (5Department of Cytogenetic, University Hospital of Caen, Caen, France) E Emmanuel Raffoux C Céline Berthon (29Service d’Hématologie, Centre Hospitalier Universitaire de Lille, Hôpital Claude-Huriez, Lille, France) L Laurene Fenwarth (Unité Mixte de Recherche (UMR) 9020-UMR-S 1277-Canther-Cancer Heterogeneity, Plasticity and Resistance to Therapies, Institut de Recherche contre le Cancer de Lille, University of Lille, CNRS, Inserm, Centre Hospitalier Universitaire Lille, Lille, France) V Vincent Marmouset (8Department of Hematology, Gustave Roussy, Université Paris-Saclay, Villejuif, France) C Cécile Pautas (4Department of Hematology, Hôpital Henri Mondor, Assistance Publique-Hôpitaux de Paris, Créteil, France) T Thomas Cluzeau (15Service d’Hématologie, Centre Hospitalier Universitaire de Nice, Nice, France) D Delphine Lebon (10CHU Amiens Picardie, Unité d'Hématologie clinique et Thérapie Cellulaire, Amiens, France) M Madalina Uzunov (11Sorbonne Université, AP-HP, Pitié- Salpêtrière Hospital, Department of Clinical Haematology, Paris, France) P Patricio Pereyra (11CABA – Alexander Fleming Institute, Olivos, Argentina) M Maël Heiblig (9Department of Hematology, Centre Hospitalier Universitaire de Lyon, Lyon, France) J Jean-Valère Malfuson (24Hôpital d'Instruction des Armées Percy, Clamart, Clamart, France) L Laure Farnault (28Service d’Hématologie, Assistance Publique-Hôpitaux de Marseille–Hôpital de la Conception, Marseille, France) P Pierre-Yves Dumas J Julia HIEULLE (2CH ROUBAIX, ROUBAIX, France) S Sarah Bertoli S Sylvain Chantepie (10Department of Hematology, Centre Hospitalier Universitaire de Caen, Caen, France) N Nathalie Auger (Department of Social and Preventive Medicine, School of Public Health, University of Montreal, QC, Canada (N.A.).) A Audrey Bidet (21Laboratory of Hematology, Bordeaux University Hospital, Bordeaux, France) M Marie Joelle Mozziconacci (22Laboratory of Hematology, Institut Paoli Calmettes, Marseille, France) A Augustin Boudry (1Laboratory of Hematology, Centre Hospitalier Universitaire de Lille, Université de Lille, INSERM U1366, Lille, France) I Isabelle Luquet (26Laboratory of Hematology, Centre Hospitalier Universitaire de Toulouse, Toulouse, France) L Laureen Chat (1Leukemia Institute Paris Saint-Louis, Paris, France) R Romain Loyaux (25Department of Biologie et pathologie médicales, Gustave Roussy, Université Paris-Saclay, Villejuif, France) L Lina EL-MURR (8Department of Hematology, Gustave Roussy, Université Paris-Saclay, Villejuif, France) E Emmanuelle Clappier (2Institut de Recherche Saint-Louis, Université Paris Cité, Paris, France) C Claude Gardin (Hématologie Clinique, Hôpital Avicenne, AP-HP, Hôpitaux Universitaires Paris Seine Saint-Denis, Bobigny, France) M Mathilde Hunault (2Department of Hematology, Centre Hospitalier Universitaire d’Angers, INSERM, Centre National de la Recherche Scientifique, Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers, Fédération Hospitalo-Universitaire Grand Ouest Against Leukemia, Université d’Angers, Université de Nantes, Angers, France) S Stéphane de Botton A Arnaud Pigneux D Dominique Penther (27Laboratory of Genetics, Centre Henri Becquerel, Rouen, France) Éric Delabesse (26Laboratory of Hematology, Centre Hospitalier Universitaire de Toulouse, Toulouse, France) R Raphaël Itzykson (6Department of Hematology, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris, Université Paris Cité, Paris, France) C Claude Preudhomme (Unité Mixte de Recherche (UMR) 9020-UMR-S 1277-Canther-Cancer Heterogeneity, Plasticity and Resistance to Therapies, Institut de Recherche contre le Cancer de Lille, University of Lille, CNRS, Inserm, Centre Hospitalier Universitaire Lille, Lille, France) H Hervé Dombret C Christian Récher N Nicolas Duployez (Unité Mixte de Recherche (UMR) 9020-UMR-S 1277-Canther-Cancer Heterogeneity, Plasticity and Resistance to Therapies, Institut de Recherche contre le Cancer de Lille, University of Lille, CNRS, Inserm, Centre Hospitalier Universitaire Lille, Lille, France) J Jean-Baptiste Micol (28Gustave-Roussy, université Paris-Saclay, Department of Hematology, Villejuif, France)

Abstract

Abstract Acute myeloid leukemia (AML) harboring NUP98 rearrangements (NUP98r) is recognized as a distinct entity in the 2022 WHO classification; however, it is not as a prognostic factor within the ELN 2022 classification. We report a large cohort of 95 adult patients with NUP98r AML. Patient characteristics included a young age (median 50 years [IQR 38-64]), 20% of therapy-related AML, a high WBC count (median 52×109/L), normal karyotype in 32%, FLT3-ITD in 48% and WT1 mutations in 34%. NUP98::NSD1 fusion was the most common (54%), and these patients were significantly younger (41y vs 61y), had more de novo AML (94% vs 64%), higher rates of normal karyotypes (56% vs 4.5%), FLT3-ITD (76% vs 18%) and WT1 mutations (50% vs 16%) than other NUP98r AML. The median overall survival (OS) for the entire cohort was 14.8 months (95% CI, 11.9–20.8) and event-free survival was 3.3 months (2-7.5). Among patients treated intensively (n=73), age (HR = 1.04) and FLT3 inhibitor therapy (HR = 0.45) influenced OS in univariate analysis, while leukocytosis, partner type, ELN classification, presence of a FLT3-ITD or WT1 mutation or hematopoietic stem cell transplant did not. Compared with NUP98 wild-type (WT) AML, NUP98r patients had a prognosis more similar to that of NUP98 WT ELN adverse patients whether initially classified as intermediate (20.3 months [11.7-30.2]) or adverse (15.7 months [13.5-42.9]). However, treatment with FLT3 inhibitors improved prognosis, with OS approaching that of intermediate-risk AML patients (33.3 months [11.9–not reached]).

Article Details

Journal Blood
Volume / Issue Vol. 146, Issue Supplement 1
Published November 03, 2025
Pages 7002-7002
ISSN 0006-4971
Publisher Elsevier BV

Journal Info

Blood

Elsevier BV

ISSN: 0006-4971 Health Sciences

Authors (41)

D

Ducourneau Benoit

3CH VALENCIENNES, VALENCIENNES, France

A

Arnaud Pages

4Gustave Roussy Cancer Center, Department of Biostatistics and Epidemiology, villejuif, France

S

Stéphanie Struski

4Department of Hematology, University Hospital of Toulouse, Toulouse, France

M

Matthieu Decamp

5Department of Cytogenetic, University Hospital of Caen, Caen, France

E

Emmanuel Raffoux

C

Céline Berthon

29Service d’Hématologie, Centre Hospitalier Universitaire de Lille, Hôpital Claude-Huriez, Lille, France

L

Laurene Fenwarth

Unité Mixte de Recherche (UMR) 9020-UMR-S 1277-Canther-Cancer Heterogeneity, Plasticity and Resistance to Therapies, Institut de Recherche contre le Cancer de Lille, University of Lille, CNRS, Inserm, Centre Hospitalier Universitaire Lille, Lille, France

V

Vincent Marmouset

8Department of Hematology, Gustave Roussy, Université Paris-Saclay, Villejuif, France

C

Cécile Pautas

4Department of Hematology, Hôpital Henri Mondor, Assistance Publique-Hôpitaux de Paris, Créteil, France

T

Thomas Cluzeau

15Service d’Hématologie, Centre Hospitalier Universitaire de Nice, Nice, France

D

Delphine Lebon

10CHU Amiens Picardie, Unité d'Hématologie clinique et Thérapie Cellulaire, Amiens, France

M

Madalina Uzunov

11Sorbonne Université, AP-HP, Pitié- Salpêtrière Hospital, Department of Clinical Haematology, Paris, France

P

Patricio Pereyra

11CABA – Alexander Fleming Institute, Olivos, Argentina

M

Maël Heiblig

9Department of Hematology, Centre Hospitalier Universitaire de Lyon, Lyon, France

J

Jean-Valère Malfuson

24Hôpital d'Instruction des Armées Percy, Clamart, Clamart, France

L

Laure Farnault

28Service d’Hématologie, Assistance Publique-Hôpitaux de Marseille–Hôpital de la Conception, Marseille, France

P

Pierre-Yves Dumas

J

Julia HIEULLE

2CH ROUBAIX, ROUBAIX, France

S

Sarah Bertoli

S

Sylvain Chantepie

10Department of Hematology, Centre Hospitalier Universitaire de Caen, Caen, France

N

Nathalie Auger

Department of Social and Preventive Medicine, School of Public Health, University of Montreal, QC, Canada (N.A.).

A

Audrey Bidet

21Laboratory of Hematology, Bordeaux University Hospital, Bordeaux, France

M

Marie Joelle Mozziconacci

22Laboratory of Hematology, Institut Paoli Calmettes, Marseille, France

A

Augustin Boudry

1Laboratory of Hematology, Centre Hospitalier Universitaire de Lille, Université de Lille, INSERM U1366, Lille, France

I

Isabelle Luquet

26Laboratory of Hematology, Centre Hospitalier Universitaire de Toulouse, Toulouse, France

L

Laureen Chat

1Leukemia Institute Paris Saint-Louis, Paris, France

R

Romain Loyaux

25Department of Biologie et pathologie médicales, Gustave Roussy, Université Paris-Saclay, Villejuif, France

L

Lina EL-MURR

8Department of Hematology, Gustave Roussy, Université Paris-Saclay, Villejuif, France

E

Emmanuelle Clappier

2Institut de Recherche Saint-Louis, Université Paris Cité, Paris, France

C

Claude Gardin

Hématologie Clinique, Hôpital Avicenne, AP-HP, Hôpitaux Universitaires Paris Seine Saint-Denis, Bobigny, France

M

Mathilde Hunault

2Department of Hematology, Centre Hospitalier Universitaire d’Angers, INSERM, Centre National de la Recherche Scientifique, Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers, Fédération Hospitalo-Universitaire Grand Ouest Against Leukemia, Université d’Angers, Université de Nantes, Angers, France

S

Stéphane de Botton

A

Arnaud Pigneux

D

Dominique Penther

27Laboratory of Genetics, Centre Henri Becquerel, Rouen, France

Éric Delabesse

26Laboratory of Hematology, Centre Hospitalier Universitaire de Toulouse, Toulouse, France

R

Raphaël Itzykson

6Department of Hematology, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris, Université Paris Cité, Paris, France

C

Claude Preudhomme

Unité Mixte de Recherche (UMR) 9020-UMR-S 1277-Canther-Cancer Heterogeneity, Plasticity and Resistance to Therapies, Institut de Recherche contre le Cancer de Lille, University of Lille, CNRS, Inserm, Centre Hospitalier Universitaire Lille, Lille, France

H

Hervé Dombret

C

Christian Récher

N

Nicolas Duployez

Unité Mixte de Recherche (UMR) 9020-UMR-S 1277-Canther-Cancer Heterogeneity, Plasticity and Resistance to Therapies, Institut de Recherche contre le Cancer de Lille, University of Lille, CNRS, Inserm, Centre Hospitalier Universitaire Lille, Lille, France

J

Jean-Baptiste Micol

28Gustave-Roussy, université Paris-Saclay, Department of Hematology, Villejuif, France