Chiroptical Signal Inversion of Peptido‐Coassemblies in Confined Parallel‐Laminar Microfluidics

J Jianjian Zhao (State Key Laboratory of Petroleum Molecular & Process Engineering Shanghai Key Laboratory of Green Chemistry and Chemical Processes Shanghai Frontiers Science Center of Molecule Intelligent Syntheses School of Chemistry and Molecular Engineering East China Normal University 3663 N. Zhongshan Road Shanghai 200062 P.R. China) W Wei‐Tao Dou (State Key Laboratory of Petroleum Molecular & Process Engineering Shanghai Key Laboratory of Green Chemistry and Chemical Processes Shanghai Frontiers Science Center of Molecule Intelligent Syntheses Liwa Institute of Skin Health, Wuhu Hospital Affiliated to East China Normal University, School of Chemistry and Molecular Engineering East China Normal University Shanghai 200062 P. R. China) W Wanding Cui (State Key Laboratory of Petroleum Molecular & Process Engineering Shanghai Key Laboratory of Green Chemistry and Chemical Processes Shanghai Frontiers Science Center of Molecule Intelligent Syntheses School of Chemistry and Molecular Engineering East China Normal University 3663 N. Zhongshan Road Shanghai 200062 P.R. China) X Xueliang Shi (East China Normal University , , ,) X Xiaodong Li (Hefei National Research Center for Physical Sciences at the Microscale, State Key Laboratory of Precision and Intelligent Chemistry) J Junfeng Fang X Xuhong Qian (State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China) H Hai‐Bo Yang (School of Chemistry and Molecular Engineering East China Normal University Shanghai China) L Lin Xu (Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, USA.)

Abstract

Abstract Chirality plays a vital role in material properties, and precise control of chiral signals is key to designing functional materials. Supramolecular self‐assembly offers an efficient means to integrate chiral building blocks with chromophores, yet controlling the assembly pathway remains challenging due to the complexity of non‐covalent interactions. Here, we introduce a continuous parallel‐laminar‐assisted self‐assembly strategy that exploits solvent ordering and solute diffusion in confined environments to regulate chiral signals in multi‐component peptide co‐assemblies. Notably, six nonpolar amino acids exhibit significantly enhanced chiroptical responses, as confirmed by circular dichroism (CD) and circularly polarized luminescence (CPL) spectroscopy. Intriguingly, Fmoc‐Ala and 1‐aminopyrene ( AP ) co‐assemblies formed in a microfluidic chip show a reversed chiroptical signal compared to those from batch reactions. Molecular dynamics (MD) simulations and COMSOL modeling suggest that velocity gradients and shear forces in microfluidics induce ordered non‐covalent interactions, altering excimer stacking and modulating chiroptical properties. This study presents an effective strategy for controlling chiral optical signals in confined environments, offering an interesting approach for supramolecular chiral transfer and regulation.

Article Details

Volume / Issue Vol. 64, Issue 27
Published July 01, 2025
ISSN 1433-7851
Publisher Wiley

Journal Info

Angewandte Chemie International Edition

Wiley

ISSN: 1433-7851 Physical Sciences

Authors (9)

J

Jianjian Zhao

State Key Laboratory of Petroleum Molecular & Process Engineering Shanghai Key Laboratory of Green Chemistry and Chemical Processes Shanghai Frontiers Science Center of Molecule Intelligent Syntheses School of Chemistry and Molecular Engineering East China Normal University 3663 N. Zhongshan Road Shanghai 200062 P.R. China

W

Wei‐Tao Dou

State Key Laboratory of Petroleum Molecular & Process Engineering Shanghai Key Laboratory of Green Chemistry and Chemical Processes Shanghai Frontiers Science Center of Molecule Intelligent Syntheses Liwa Institute of Skin Health, Wuhu Hospital Affiliated to East China Normal University, School of Chemistry and Molecular Engineering East China Normal University Shanghai 200062 P. R. China

W

Wanding Cui

State Key Laboratory of Petroleum Molecular & Process Engineering Shanghai Key Laboratory of Green Chemistry and Chemical Processes Shanghai Frontiers Science Center of Molecule Intelligent Syntheses School of Chemistry and Molecular Engineering East China Normal University 3663 N. Zhongshan Road Shanghai 200062 P.R. China

X

Xueliang Shi

East China Normal University , , ,

X

Xiaodong Li

Hefei National Research Center for Physical Sciences at the Microscale, State Key Laboratory of Precision and Intelligent Chemistry

J

Junfeng Fang

X

Xuhong Qian

State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China

H

Hai‐Bo Yang

School of Chemistry and Molecular Engineering East China Normal University Shanghai China

L

Lin Xu

Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, USA.