Children’s state anxiety before MRI scanning and resting state functional connectivity in large scale brain networks

Abstract

Abstract Introduction Most resting-state functional connectivity (rs-FC) research does not consider the participant’s subjective state during magnetic resonance imaging (MRI). Heightened anxiety before an MRI (“pre-scanning state anxiety”) may influence rs-FC and complicate interpretation of individual differences, particularly in underrepresented groups whose scanning experiences may differ from typical research samples. Methods We assessed associations between pre-scanning state anxiety and rs-FC within and between the default mode network (DMN) and salience network in a trait-anxious community sample of Latina girls (8–13 years) and a companion sample of treatment-seeking and healthy youth (8–18 years) of predominantly non-Latinx background. A constrained network-based statistical approach calculated the average of un-thresholded correlation coefficients from edge-level partial Spearman correlations to produce network-level measures (7 cortical + 1 subcortical). This approach is “constrained” in that analyses operate at the spatial scale of functional networks, rather than individual edges, to increase statistical power. Statistics were compared against a permutation-based null distribution to assess significance (Bonferroni corrected p  < 0.00139). Results Reduced rs-FC within the DMN ( r  = − 0.32, p  < 0.00139) was associated with pre-scanning state anxiety in the community sample, but did not replicate in our companion sample. Discussion Pre-scanning state anxiety is associated with rs-FC within the DMN, but only among a trait-anxious community sample. Individual differences in MRI scanning experiences may be associated with rs-FC, but sample characteristics and replication should be considered.