Biosynthesis of the Red Algal Diterpene Peyssonnosol in Bacteria
Abstract
Abstract Two diterpene synthases (DTSs) for peyssonnosol and peyssonnosol B were discovered in bacteria. Their enzyme mechanisms were investigated through isotopic labelling experiments, density functional theory (DFT) calculations and site‐directed mutagenesis, yielding biosynthetically related molecules that gave important insights into the terpene cyclisation cascade. In the present case, several mechanisms in line with the isotopic labelling experiments could be formulated. Only an extended experimental approach in combination with computational chemistry ultimately resulted in a refined mechanistic model.
Article Details
Authors (11)
Zhiyong Yin
Kekulé-Institute for Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagk-Straße 1, Bonn 53121, Germany
Zhehui Hu
State Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Center of Materials Synthetic Biology
Kexin Yang
School of Life Science and Technology, ShanghaiTech University
Guihu Lu
State Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Center of Materials Synthetic Biology
Georges B. Tabekoueng
Kekulé Institute of Organic Chemistry and Biochemistry University of Bonn Gerhard‐Domagk‐Straße 1 53121 Bonn Germany
Pingping Wu
Qi Luo
Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, School of Traditional Chinese Medicine
Juan Xu
Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, School of Ecological and Environmental Sciences
Bernd Goldfuss
Institute of Organic Chemistry, Department of Chemistry, University of Cologne, Greinstraße 4, 50939 Cologne, Germany
Jeroen S. Dickschat
Kekulé Institute for Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagk-Straße 1, 53121 Bonn, Germany
Guangkai Bian
State Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Center of Materials Synthetic Biology