Bioremediation of lunar regolith simulant through mycorrhizal fungi and plant symbioses enables chickpea to seed

Abstract

Abstract Food sustainability is a significant challenge for long-term space travel. Plants can provide fresh nutrition, reducing reliance on packaged foods. Using Lunar regolith simulant (LRS), we tested a methodology to create a productive growth medium for horticultural crops on the Moon. We leveraged chickpea ( Cicer arietinum ), Arbuscular Mycorrhizal Fungi (AMF), and Vermicompost (VC) to enhance plant stress tolerance, sequester contaminants, and improve substrate structure. Chickpeas were cultivated in LRS/VC mixtures, with or without AMF, under climate-controlled conditions. Plants seeded successfully in mixtures containing up to 75% LRS when inoculated with AMF. While the number of seeds declined with increasing LRS concentration, seed size remained stable. Higher LRS concentrations induced stress; however, plants grown in 100% LRS inoculated with AMF demonstrated an average extension of two weeks in survival compared to non-inoculated plants. AMF colonized roots across all mixtures, including 100% LRS, demonstrating the ability to establish symbioses under extreme conditions. We also observed improvement in the structural properties of LRS by forming aggregates capable of withstanding extreme conditions, potentially mitigating particle-related hazards. These results provide a baseline for chickpea establishment and yield in amended LRS while demonstrating biological improvements in regolith properties.