Biomimetic Total Synthesis and Paired Omics Identify an Intermolecular Diels–Alder Reaction as the Key Step in Lugdunomycin Biosynthesis

Michiel T. Uiterweerd

Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 7, Groningen 9747 AG, The Netherlands

Isabel Nuñez Santiago

Institute of Biology, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands

Ana V. Cunha

Faculty of Engineering, University of Antwerp, IPRACS, Groenenborgerlaan 171, Antwerpen 2020, Belgium

Remco W. A. Havenith

Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 7, Groningen 9747 AG, The Netherlands

Chao Du

Institute of Biology, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands

Le Zhang

Helga U. van der Heul

Institute of Biology, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands

Somayah S. Elsayed

Institute of Biology, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands

Adriaan J. Minnaard

Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 7, Groningen 9747 AG, The Netherlands

Gilles P. van Wezel

Institute of Biology, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands