Azacitidine to treat measurable residual disease in patients with MDS/AML: final long-term results of the RELAZA2 trial

A Anne Sophie Platzbecker (1Else Kroener Fresenius Center for Digital Health, Faculty of Medicine and University Hospital Carl Gustav Carus, Dresden University of Technology, Dresden, Germany) J Julia-Annabell Georgi (4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany) J Jan Moritz Middeke K Katja Sockel (4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany) R Rebekka Wehner R Regina Herbst (7Department III of Internal Medicine, Klinikum Chemnitz, Chemnitz, Germany) D Dominik Wolf C Claudia D. Baldus U Uta Oelschlägel (4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany) A Anke Mütherig (4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany) L Lars Fransecky (14Department of Internal Medicine II, University Hospital Schleswig Holstein, Campus Kiel, Kiel, Germany) R Richard Noppeney (11Division of Hematology/Oncology, Medizinische Klinik I, Krankenhaus der Barmherzigen Brüder, Trier, Germany) G Gesine Bug (13Department of Medicine 2, University Hospital, Goethe University Frankfurt, Frankfurt, Germany) K Katharina S. Götze A Alwin Krämer T Tilmann Bochtler M Matthias Stelljes (16Department of Medicine A, University Hospital Münster, Münster, Germany) E Eva Eßeling (19Department of Medicine A, Hematology, Oncology, and Respiratory Medicine, University Hospital Münster, Münster, Germany) F Friedrich Stölzel (8Department of Internal Medicine II, Hematology and Oncology, University Medical Center Schleswig-Holstein, Campus Kiel, Kiel, Germany) M Malte von Bonin (4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany) H Hubert Serve M Mathias Hänel (7Department III of Internal Medicine, Klinikum Chemnitz, Chemnitz, Germany) U Ulrich Dührsen A Anna Harig (4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany) C Carsten Müller-Tidow J Johannes Schetelig (4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany) S Sebastian Stasik (4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany) C Christoph Röllig (22Department of Internal Medicine I, University Hospital TU Dresden, Dresden, Germany) G Gerhard Ehninger (4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany) M Michael Krämer M Marc Schmitz M Martin Bornhäuser U Uwe Platzbecker C Christian Thiede (7University Hospital, Dresden University of Technology, Dresden, Germany)

Abstract

Abstract Measurable residual disease (MRD) can predict relapse in patients with advanced myelodysplastic neoplasms (MDS) or acute myeloid leukemia (AML). We report the long-term efficacy and safety of MRD-guided preemptive azacitidine treatment to prevent relapse in the phase 2 Relapse Prevention With Azacitidine (RELAZA2) trial. Patients with MDS or AML after either intensive chemotherapy only or consecutive allogeneic stem cell transplantation were prospectively screened for imminent relapse by molecular MRD assessment. Patients who became MRD positive (MRDpos) during screening received azacitidine for up to 2 years to prevent relapse. The primary end point was the proportion of patients alive and relapse-free 6 months after azacitidine start. Of 357 patients screened, 119 (33.3%) became MRDpos, of whom 95 (79.8%) were eligible for azacitidine treatment. The primary end point was met; 60 (63%) patients were relapse free (95% confidence interval, 54-71; P< .0001) 6 months after azacitidine initiation with no new safety signals. Of 60 patients achieving MRD response during the first 6 cycles of azacitidine, 31 (52%) maintained response without hematological relapse for ≥2 years after azacitidine initiation. The median treatment-free duration after azacitidine discontinuation was 20.8 months; the longest ongoing response was 104 months. After a median follow-up of 6.6 years, 15 initial responders (25%) remained alive and in remission. Among screened patients who remained continuously MRD negative, 60-month overall survival and relapse-free survival were 88% and 79%, respectively. Patients with continuously negative MRD display a very favorable prognosis. Most patients with MRD positivity can be effectively treated with azacitidine with potential long-term remission even after termination of azacitidine. This trial was registered at www.clinicaltrials.gov as #NCT01462578.

Article Details

Journal Blood
Volume / Issue Vol. 147, Issue 10
Published March 05, 2026
Pages 1098-1110
ISSN 0006-4971
Publisher Elsevier BV

Journal Info

Blood

Elsevier BV

ISSN: 0006-4971 Health Sciences

Authors (34)

A

Anne Sophie Platzbecker

1Else Kroener Fresenius Center for Digital Health, Faculty of Medicine and University Hospital Carl Gustav Carus, Dresden University of Technology, Dresden, Germany

J

Julia-Annabell Georgi

4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany

J

Jan Moritz Middeke

K

Katja Sockel

4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany

R

Rebekka Wehner

R

Regina Herbst

7Department III of Internal Medicine, Klinikum Chemnitz, Chemnitz, Germany

D

Dominik Wolf

C

Claudia D. Baldus

U

Uta Oelschlägel

4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany

A

Anke Mütherig

4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany

L

Lars Fransecky

14Department of Internal Medicine II, University Hospital Schleswig Holstein, Campus Kiel, Kiel, Germany

R

Richard Noppeney

11Division of Hematology/Oncology, Medizinische Klinik I, Krankenhaus der Barmherzigen Brüder, Trier, Germany

G

Gesine Bug

13Department of Medicine 2, University Hospital, Goethe University Frankfurt, Frankfurt, Germany

K

Katharina S. Götze

A

Alwin Krämer

T

Tilmann Bochtler

M

Matthias Stelljes

16Department of Medicine A, University Hospital Münster, Münster, Germany

E

Eva Eßeling

19Department of Medicine A, Hematology, Oncology, and Respiratory Medicine, University Hospital Münster, Münster, Germany

F

Friedrich Stölzel

8Department of Internal Medicine II, Hematology and Oncology, University Medical Center Schleswig-Holstein, Campus Kiel, Kiel, Germany

M

Malte von Bonin

4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany

H

Hubert Serve

M

Mathias Hänel

7Department III of Internal Medicine, Klinikum Chemnitz, Chemnitz, Germany

U

Ulrich Dührsen

A

Anna Harig

4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany

C

Carsten Müller-Tidow

J

Johannes Schetelig

4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany

S

Sebastian Stasik

4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany

C

Christoph Röllig

22Department of Internal Medicine I, University Hospital TU Dresden, Dresden, Germany

G

Gerhard Ehninger

4Department of Internal Medicine I, University Hospital Dresden, Dresden University of Technology, Dresden, Germany

M

Michael Krämer

M

Marc Schmitz

M

Martin Bornhäuser

U

Uwe Platzbecker

C

Christian Thiede

7University Hospital, Dresden University of Technology, Dresden, Germany