Association of NUDT17 rs9286836 and rs2004659 variants with breast cancer risk in Bangladeshi Women

Abstract

Breast cancer is a multifaceted illness impacted by genetic factors as well as environmental influences. While variants in the NUDT17 gene have been associated with cancer biology, their involvement in breast cancer is still inadequately investigated, especially within South Asian populations. This study investigated the association between two NUDT17 polymorphisms, rs9286836 and rs2004659, and breast cancer risk in Bangladeshi women using a case–control design. A total of 240 breast cancer patients and 240 age-, sex-, and BMI-matched healthy controls were enrolled. Genomic DNA was extracted from blood samples, and genotyping was performed using tetra-primer ARMS-PCR for rs9286836 and PCR–RFLP for rs2004659. The statistical methods employed included chi-square tests for genotype distributions and logistic regression to calculate odds ratios (ORs) with 95% confidence intervals (CIs). Genotyping for NUDT17 rs9286836 was successfully completed for all recruited participants. However, for rs2004659, high-quality genotyping data were available for 204 breast cancer cases and 204 controls after quality control procedures. No significant association was observed between rs9286836 and breast cancer risk across the tested genetic models. In contrast, rs2004659 showed a robust protective association with breast cancer, particularly among heterozygous individuals, and this protective effect was consistently observed across the dominant, over-dominant, and allelic models. Haplotype analysis revealed that the AA haplotype was associated with an increased risk of breast cancer, whereas the AG and GA haplotypes were associated with a reduced risk. Expression analysis further demonstrated elevated NUDT17 levels in breast cancer tissues and genotype-dependent expression effects for both variants. These findings suggest a protective role of rs2004659 in breast cancer susceptibility and highlight the potential of NUDT17 polymorphisms as biomarkers in Bangladeshi women.