Archaeogenetic insights into the demographic history of Late Neanderthals

C Charoula M. Fotiadou (Archaeo- and Palaeogenetics, Institute for Archaeological Sciences, Department of Geosciences, University of Tübingen) J Jesper Borre Pedersen (Research Center “The Role of Culture in Early Expansions of Humans (ROCEEH)” of the Heidelberg Academy of Sciences and Humanities, University of Tübingen) H Hélène Rougier M Mirjana Roksandic (Natural Sciences and Engineering Research Council of Canada, Canada Research Chair in Human Evolution, Department of Anthropology, University of Winnipeg) M Maria A. Spyrou (Archaeo- and Palaeogenetics, Institute for Archaeological Sciences, Department of Geosciences, University of Tübingen) K Kathrin Nägele (Department of Archaeogenetics, Max Planck Institute for Evolutionary Anthropology) E Ella Reiter H Hervé Bocherens A Andrew W. Kandel M Miriam N. Haidle (Research Center “The Role of Culture in Early Expansions of Humans (ROCEEH)” of the Heidelberg Academy of Sciences and Humanities, University of Tübingen) T Timo P. Streicher (Research Center “The Role of Culture in Early Expansions of Humans (ROCEEH)” of the Heidelberg Academy of Sciences and Humanities, University of Tübingen) N Nicholas J. Conard (Senckenberg Centre for Human Evolution and Palaeoenvironment at the University of Tübingen) F Flora Schilt (Department of Art and Culture, History and Antiquity, Vrije Universiteit Amsterdam) R Ricardo Miguel Godinho (Interdisciplinary Center for Archaeology and the Evolution of Human Behavior (ICArEHB), Faculdade das Ciências Humanas e Sociais, Universidade do Algarve (UAlg), Campus de Gambelas) T Thorsten Uthmeier (Department of Geosciences, University of Arizona) L Luc Doyon P Patrick Semal J Johannes Krause A Alvise Barbieri (Department of Art and Culture, History and Antiquity, Vrije Universiteit Amsterdam) D Dušan Mihailović (Department of Archaeology, Faculty of Philosophy, University of Belgrade) I Isabelle Crevecoeur C Cosimo Posth

Abstract

The demographic history of Neanderthals is only partially understood. In Europe, some degree of genetic continuity has been shown from 120 thousand years ago (ka) onward despite the occurrence of multiple subsequent diversification events. While it has been proposed that a population turnover preceded the emergence of Late Neanderthals in Europe, the extent, timing, and geographic location of this event are currently unknown. Here, we report ten mitochondrial DNA sequences (mtDNAs) of Neanderthal individuals from six archaeological sites in Belgium, France, Germany and Serbia, and analyze them alongside 49 published mtDNAs. The integration of phylogenetic and molecular dating analyses with an extensive archaeological dataset enabled us to reconstruct temporal and spatial patterns in Neanderthal distribution. Remarkably, nearly all Late Neanderthal individuals across Europe belong to a single mtDNA lineage that diversified recently, confirming a large-scale genetic replacement. Our analyses date this diversification event to approximately 65 ka and suggest that it likely originated from a population refugium in southwestern France from which Neanderthals appear to have undergone a major range dispersal across Europe. In addition, we detect a sharp decline in the Neanderthal mtDNA effective population size beginning ~45 ka and reaching a minimum ~42 ka, shortly before their extinction. This study demonstrates that integrating molecular and archaeological datasets provides a more detailed understanding of the Late Neanderthal population’s history, and highlights the critical role of climate-driven refugia and subsequent range expansions in shaping the genetic landscape of Neanderthals through time.

Article Details

Volume / Issue Vol. 123, Issue 13
Published March 31, 2026
ISSN 0027-8424
Publisher National Academy of Sciences

Authors (22)

C

Charoula M. Fotiadou

Archaeo- and Palaeogenetics, Institute for Archaeological Sciences, Department of Geosciences, University of Tübingen

J

Jesper Borre Pedersen

Research Center “The Role of Culture in Early Expansions of Humans (ROCEEH)” of the Heidelberg Academy of Sciences and Humanities, University of Tübingen

H

Hélène Rougier

M

Mirjana Roksandic

Natural Sciences and Engineering Research Council of Canada, Canada Research Chair in Human Evolution, Department of Anthropology, University of Winnipeg

M

Maria A. Spyrou

Archaeo- and Palaeogenetics, Institute for Archaeological Sciences, Department of Geosciences, University of Tübingen

K

Kathrin Nägele

Department of Archaeogenetics, Max Planck Institute for Evolutionary Anthropology

E

Ella Reiter

H

Hervé Bocherens

A

Andrew W. Kandel

M

Miriam N. Haidle

Research Center “The Role of Culture in Early Expansions of Humans (ROCEEH)” of the Heidelberg Academy of Sciences and Humanities, University of Tübingen

T

Timo P. Streicher

Research Center “The Role of Culture in Early Expansions of Humans (ROCEEH)” of the Heidelberg Academy of Sciences and Humanities, University of Tübingen

N

Nicholas J. Conard

Senckenberg Centre for Human Evolution and Palaeoenvironment at the University of Tübingen

F

Flora Schilt

Department of Art and Culture, History and Antiquity, Vrije Universiteit Amsterdam

R

Ricardo Miguel Godinho

Interdisciplinary Center for Archaeology and the Evolution of Human Behavior (ICArEHB), Faculdade das Ciências Humanas e Sociais, Universidade do Algarve (UAlg), Campus de Gambelas

T

Thorsten Uthmeier

Department of Geosciences, University of Arizona

L

Luc Doyon

P

Patrick Semal

J

Johannes Krause

A

Alvise Barbieri

Department of Art and Culture, History and Antiquity, Vrije Universiteit Amsterdam

D

Dušan Mihailović

Department of Archaeology, Faculty of Philosophy, University of Belgrade

I

Isabelle Crevecoeur

C

Cosimo Posth