Achieving Near‐Infrared Organic Room‐Temperature Phosphorescence for High‐Resolution Immune Response Monitoring and Bioimaging
Abstract
Abstract Organic near‐infrared (NIR) room‐temperature phosphorescent (RTP) materials hold great potential for bioimaging due to their ability to eliminate background noise and tissue autofluorescence. Here, we synthesized octa‐ring fused RTP molecules (TPP‐BN and TPP‐BF) with B─N coordination bonds via a two‐step reaction, enabling NIR phosphorescent emission at 819 nm and a 28.6 ms lifetime. Using PMMA‐b‐PEG as host and surfactant to stabilize the RTP molecules, we fabricated PMMA‐b‐PEG based nanoparticles (PNPs) with five‐fold brighter afterglow than conventional F127‐based methods (FNPs). We further developed a granzyme B (GrB)‐responsive nanoprobe (Q‐BFNP) that achieves specific and quantitative detection. In vivo studies demonstrated their ability to monitor and distinguish tumor immune response with the signal‐to‐background ratio (SBR) as high as 216.4. This study provides a new method for constructing NIR organic RTP probes and advances applications of RTP materials in real‐time, high‐contrast bioimaging and tumor immune monitoring.
Article Details
Authors (18)
Yeyun Zhao
Xianbin Ma
School of Medical Technology Beijing Institute of Technology Beijing 100081 P.R. China
Shisheng Cui
Beijing Key Laboratory of Construction Tailorable Advanced Functional Materials and Green Applications School of Materials Science and Engineering Beijing Institute of Technology Beijing 100081 P.R. China
Jiamin Qu
Beijing Key Laboratory of Construction Tailorable Advanced Functional Materials and Green Applications School of Materials Science and Engineering Beijing Institute of Technology Beijing 100081 P.R. China
Yuqi Wen
Kai Zhang
Gengchen Li
Yongfeng Zhang
Xiaoyuan Huang
Baicheng Mei
Department of Materials Science and Engineering, University of Illinois 1 , Urbana, Illinois 61801,
Tao Wang
Peng Sun
State Key Laboratory of NBC Protection for Civilian
Jianbing Shi
Bin Tong
Hai‐Yan Xie
School of Pharmaceutical Sciences Peking University Beijing 100191 P.R. China
Zitong Liu
State Key Laboratory of Natural Product Chemistry, Key Laboratory of Special Function Materials and Structure Design (MOE), College of Chemistry and Chemical Engineering
Zhengxu Cai
Yuping Dong