Abstract 4364855: Linking <i>In Vivo</i> Flap Motion to <i>Ex Vivo</i> Flap Thickness in Type B Aortic Dissection: Acute vs Chronic Insights

Abstract

Introduction: Type B aortic dissection (TBAD) is a serious condition that may benefit from early endovascular repair. In the acute phase (aTBAD; &lt;14 days onset), the high compliance of the dissection flap improves success of endovascular repair. In the chronic phase (cTBAD; &gt;90 days onset), the flap stiffens and makes endovascular repair less effective. This study aimed to better characterize dissection flap behavior and understand the cause of stiffening. Hypothesis: We hypothesize that remodeling of the dissection flap in TBAD leads to decreased dynamic displacement over time, driven by structural thickening and stiffening. Methods: We combined in vivo imaging and ex vivo tissue analyses. A total of 15 2D phase-contrast magnetic resonance imaging (PC-MRI) datasets were analyzed (4 cTBAD; 11 aTBAD). For dissection flap thickness, a separate cohort of 41 tissue samples were used (20 cTBAD; 10 aTBAD, 11 control). We used an in-house MATLAB code to analyze flap motion in the 2D PC-MRI data. We extracted maximum displacement by calculating the Euclidean distance between corresponding points on the flap at systole and diastole. After surgical excision, dissection flap samples were placed into cryopreservation medium (10% DMSO + 90% RPMI) and stored in a -80 °C freezer. Prior to measurement, tissue was thawed at around 37 °C, and tissue thickness was measured by a micrometer. Control dissection flaps were created by peeling healthy descending aortic tissue at the level of the media and combined intimal and partial medial layers were measured together as control flap thickness. Group differences were assessed using the Mann-Whitney U test. Results: 2D PC-MRI revealed large differences in dissection flap displacement between cTBAD and aTBAD (Fig. 1A,B). Mean maximum displacements in the acute group (5.4±1.9 mm) were significantly higher than chronic (1.5±1.1 mm; p &lt; 0.01). Thickness measurements of the dissection flaps varied greatly: cTBAD = 1.8±0.4, aTBAD = 1.3±0.4, control = 0.8±0.1 mm. All group comparisons revealed significant differences (Fig. 1C). Since bending stiffness is proportional to the cube of thickness, we can also expect a significant increase in chronic dissection flap stiffness. Conclusions: Dissection flaps in chronic TBAD exhibit reduced motion and increased stiffness compared to the acute phase. These properties are related to structural thickening which highlights the importance of early intervention before adverse remodeling occurs.