A receptor-like mechanosensitive protein governs preprophase band positioning for asymmetric cell divisions and SC morphogenesis
Abstract
Asymmetric cell division underpins cellular diversity in multicellular plants. These divisions are mechanosensitive, and preprophase band (PPB) formation hinges on cell-wall mechanical properties in plant cells. Yet, the spatial control mechanism governing this process in plants remains elusive. During grass stomatal development, mechanical cues originate from differential growth rates and cell wall modifications at the interface of guard mother cell/subsidiary mother cell (SMC). In this work, we have identified a maize receptor-like protein, KAI1, that functions as a master regulator of subsidiary cell formation within the stomatal complex. KAI1 governs division-plane orientation in SMCs through mechanochemical signaling: It perceives cell wall rigidity via pectin interaction, and subsequently recruits tubulin for PPB positioning, thereby directing division-plane specification. This work uncovers a plant-unique mechanosensitive protein that mediates extracellular matrix cues to cytoskeletal reorganization during asymmetric division for cell diversity. Our findings establish that KAI1 governs a cell wall mechanics-dependent PPB positioning to control the exact division plane alignment of the SMC. This mechanism subsequently mediates the regulation of SMC polarization and subsidiary cell morphogenesis during stomatal development.
Article Details
Journal Info
Proceedings of the National Academy of Sciences
National Academy of Sciences
Authors (17)
Zhikun Duan
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University
Wenwen Duan
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University
Zihao Zhang
Shanghai Engineering Research Center of Tooth Restoration and Regeneration and Tongji Research Institute of Stomatology and Department of Implantology, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University
Kaiwen Li
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University
Suting Wang
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University
Jiapeng Yang
Chemistry and Biomedicine Innovation Center, Ministry of Education Key Laboratory of High Performance Polymer Materials and Technology, School of Chemistry and Chemical Engineering, Nanjing University
Baolin Xiao
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University
Tian Zhang
Division of Hematology‐Oncology, Department of Internal Medicine University of Texas Southwestern Medical Center Dallas Texas USA
Jianchao Ma
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University
Baozhu Li
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University
Pengtao Wang
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University
Yi Cao
David W. Galbraith
School of Plant Sciences and Bio5 Institute, The University of Arizona
Alistair M. Hetherington
School of Biological Sciences, University of Bristol
Jingjing Xing
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University
Siyi Guo
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University
Chun-Peng Song
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University