A pentatomomorpha-specific salivary protein activates plant immunity and is critical for insect feeding
Abstract
The stinkbug Riptortus pedestris , notorious for inducing soybean staygreen-like syndrome, employs a range of salivary proteins to manipulate the host plant for its benefit. Here, we show that RpSP1, a salivary protein specific to Pentatomomorpha, triggers plant defense responses in multiple plant species. RpSP1 interacts with and stabilizes a HSP40 family protein GmSPIP1 and is dependent on GmSPIP1 to induce cell death. We show that a critical 22-amino acid peptide within RpSP1 acts as an intracellular insect-derived elicitor. Furthermore, RpSP1 enhances insect-feeding efficiency. The dual functionality of RpSP1 is highlighted by the significant reduction of soybean staygreen-like syndrome following its overexpression in soybean plants or knockdown in insects. Our findings elucidate the complex molecular interactions between plants and herbivores, positioning RpSP1 as a crucial target for developing advanced pest management strategies with broad implications for agricultural biology.
Article Details
Journal Info
Proceedings of the National Academy of Sciences
National Academy of Sciences
Authors (10)
Biao Hu
School of Nuclear Science and Technology, University of South China, 28 Changsheng West Road, Hengyang 421001, China
Xiuli Feng
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs (MARA), Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University
Manru Xu
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs (MARA), Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University
Yue Huang
Chunyun Guo
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs (MARA), Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University
Ruikun Yuan
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs (MARA), Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University
Yiyuan Li
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs (MARA), Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University
Zhongyan Wei
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs (MARA), Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University
Jianping Chen
Zongtao Sun
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs (MARA), Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University