A Mucosa‐Inspired Dynamic Biointerface Engineering a Biofilm‐Preventive Niche Against Pathogenic Microbiome Dysbiosis

Abstract

ABSTRACT A stable symbiosis within the microbiome‐host axis is essential for human health. However, preventing microbiome dysbiosis using biomaterials remains challenging due to their unpredictable influence on microbiome evolution. Inspired by the defensive niche of symbiotic mucosa, we have developed a biointerface that forms an engineered mucosa‐inspired dynamic niche to prevent pathogenic dysbiosis. This biointerface features a dynamic zwitterionic network that emulates the mucosa's biophysical defensive functions. Notably, by leveraging its dynamic niche, the biointerface restricts microbial attachment and aggregation, thereby preventing biofilm formation. Comprehensive metagenomic analyses reveal that microbial communities adapt to this biofilm‐preventive dynamic biointerface compositionally and functionally. In particular, Gram‐negative bacteria were relatively reduced, along with decreased abundance of pathways associated with virulence and biofilm formation. Consequently, the mucosa‐inspired biointerface intrinsically prevents the development of pathogenic dysbiosis. This study demonstrates the groundbreaking potential of material‐based niche engineering to guide the ecological shifts of microbial communities from the material scale.