The NADH-dependent flavin reductase ThdF follows an ordered sequential mechanism though crystal structures reveal two FAD molecules in the active site - Hendrik J. Horstmeier; Simon Bork; Marius F. Nagel; Willy Keller; Jens Sproß; Niklas Diepold; Marie Ruppel; Tilman Kottke; Hartmut H. Niemann (Journal of Biological Chemistry, 2025)